Device

Part:BBa_K771001:Design

Designed by: WU Yuqi;YIN Ruonan;GUO Huaqinq   Group: iGEM12_SJTU-BioX-Shanghai   (2012-09-25)

Membrane Anchor 1 :ssDsbA-Lgt-PP7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1129
    Illegal BglII site found at 1385
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 883
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 504


Design Notes

For Membrane Switch


Source

ssDsbA, Lgt from E.coli genome; MS2 is aggregation deficient mutant of its wildtype

References

http://2012.igem.org/Team:SJTU-BioX-Shanghai/Project/project1.2 Membrane Accelerator
http://2012.igem.org/Team:SJTU-BioX-Shanghai/Project/project1.3 Membrane Rudder